Cloning and expression of cDNA encoding human betacellulin, a new member of the EGF family

Biochem Biophys Res Commun. 1993 Feb 15;190(3):1173-9. doi: 10.1006/bbrc.1993.1173.


Betacellulin (BTC) is a novel growth factor purified from the conditioned media of mouse pancreatic beta tumor cells and has been found to be a new member of the epidermal growth factor (EGF) family. The cDNA encoding human BTC has been cloned from a cDNA library prepared from human breast adenocarcinoma cell line MCF-7. The nucleotide sequence encodes a polypeptide which consists of 178 amino acid residues including a putative signal sequence, indicating that the structural organization of human BTC is similar to that of mouse BTC. The amino acid sequence of the human BTC precursor protein exhibits 79% similarity with that of the mouse precursor protein. The BTC gene was found to be expressed in several mouse tissues including kidney and liver as well as in a mouse beta tumor cell line and MCF-7 cells, suggesting that BTC might play a physiological role in normal tissues.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Betacellulin
  • Cloning, Molecular
  • DNA / genetics
  • Gene Expression
  • Growth Substances / genetics*
  • Humans
  • In Vitro Techniques
  • Intercellular Signaling Peptides and Proteins*
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • Sequence Alignment
  • Transfection
  • Tumor Cells, Cultured


  • BTC protein, human
  • Betacellulin
  • Btc protein, mouse
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • RNA, Messenger
  • DNA

Associated data

  • GENBANK/S55582
  • GENBANK/S55606
  • GENBANK/S64629
  • GENBANK/S64630
  • GENBANK/S64631
  • GENBANK/S64632
  • GENBANK/S64635
  • GENBANK/S64636
  • GENBANK/S64637
  • GENBANK/U07167