Ki-67 expression and BrdUrd incorporation as markers of proliferative activity in human prostate tumour models

Cell Prolif. 1993 Jan;26(1):67-75. doi: 10.1111/j.1365-2184.1993.tb00007.x.


The validity of the use of the monoclonal antibodies Ki-67 and anti-BrdUrd to evaluate proliferative activity of human prostate tumour models was studied. Growth of the transplantable PC-82 and PC-EW prostate tumours, as assessed by tumour volume measurements, was significantly correlated with the proliferative activity as reflected by BrdUrd incorporation into DNA (r = 0.64 and r = 0.78, respectively). The proliferative activity of PC-82 tumours detected by Ki-67 antigen expression paralleled the pattern observed with BrdUrd (r = 0.51) and a significant correlation (r = 0.60) between the results obtained with both markers was found. In growing PC-82 and PC-EW tumours only small variations in the Ki-67 and BrdUrd indices were observed. In contrast, Ki-67 expression in regressing PC-82 tumours varied considerably (2.7 +/- 2.2%). The BrdUrd index in regressing PC-82 tumours showed less variation (1.3 +/- 0.2%), but part of the BrdUrd-positive cells were found in the stromal (murine) part of the regressing tissue. It is concluded that the Ki-67 and BrdUrd proliferation markers are reliable parameters to monitor changes in growth of prostate tumour lines, but that in slow growing or regressing tumours Ki-67 and BrdUrd data should be interpreted with caution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers, Tumor / analysis*
  • Bromodeoxyuridine / metabolism*
  • Cell Division
  • Dihydrotestosterone / analysis
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Ki-67 Antigen
  • Male
  • Mice
  • Models, Biological
  • Neoplasm Proteins / biosynthesis*
  • Nuclear Proteins / biosynthesis*
  • Prostatic Neoplasms / chemistry
  • Prostatic Neoplasms / metabolism
  • Prostatic Neoplasms / pathology*
  • Testosterone / analysis
  • Testosterone / blood
  • Tumor Cells, Cultured / transplantation


  • Biomarkers, Tumor
  • Ki-67 Antigen
  • Neoplasm Proteins
  • Nuclear Proteins
  • Dihydrotestosterone
  • Testosterone
  • Bromodeoxyuridine