Assembly of a processive messenger RNA polyadenylation complex

EMBO J. 1993 Feb;12(2):585-94.

Abstract

Polyadenylation of mRNA precursors by poly(A) polymerase depends on two specificity factors and their recognition sequences. These are cleavage and polyadenylation specificity factor (CPSF), recognizing the polyadenylation signal AAUAAA, and poly(A) binding protein II (PAB II), interacting with the growing poly(A) tail. Their effects are independent of ATP and an RNA 5'-cap. Analysis of RNA-protein interactions by non-denaturing gel electrophoresis shows that CPSF, PAB II and poly(A) polymerase form a quaternary complex with the substrate RNA that transiently stabilizes the binding of poly(A) polymerase to the RNA 3'-end. Only the complex formed from all three proteins is competent for the processive synthesis of a full-length poly(A) tail.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Carrier Proteins / metabolism
  • Hydrolysis
  • Poly A / biosynthesis
  • Poly(A)-Binding Proteins
  • Polynucleotide Adenylyltransferase / metabolism*
  • RNA Processing, Post-Transcriptional*
  • RNA, Messenger / metabolism*
  • RNA-Binding Proteins / metabolism
  • Substrate Specificity
  • mRNA Cleavage and Polyadenylation Factors

Substances

  • Carrier Proteins
  • Poly(A)-Binding Proteins
  • RNA, Messenger
  • RNA-Binding Proteins
  • mRNA Cleavage and Polyadenylation Factors
  • Poly A
  • Adenosine Triphosphate
  • Polynucleotide Adenylyltransferase