Yeasts have a four-fold variation in ribosomal DNA copy number

Yeast. 1993 Jan;9(1):53-8. doi: 10.1002/yea.320090107.

Abstract

By employing pulsed-field gel electrophoresis we have determined the size of the rDNA cluster in wild-type yeast strains representing genera of Candida, Kluyveromyces, Pachysolen, Schizosaccharomyces and Torulaspora. Although the genome size of the examined species is similar (12.3-13.9 Mb), at least a four-fold variation has been observed between the lowest amount of rDNA repeats in P. tannophilus (28) and the highest in C. glabrata and S. poombe (> 115). In two species the rDNA cluster is represented by two loci, residing either in one (S. pombe) or two chromosomes (C. glabrata).

Publication types

  • Comparative Study

MeSH terms

  • Candida / genetics
  • DNA Probes
  • DNA, Fungal / genetics*
  • DNA, Ribosomal / genetics*
  • Electrophoresis, Gel, Pulsed-Field
  • Genome, Fungal*
  • Kluyveromyces / genetics
  • Schizosaccharomyces / genetics
  • Species Specificity
  • Yeasts / genetics*

Substances

  • DNA Probes
  • DNA, Fungal
  • DNA, Ribosomal