PCR amplification of SRY-related gene sequences reveals evolutionary conservation of the SRY-box motif

PCR Methods Appl. 1993 Feb;2(3):218-22. doi: 10.1101/gr.2.3.218.


SRY (sex-determining region of the Y chromosome) has recently been identified as a key regulatory gene in mammalian sex determination. The open reading frame of this gene contains an 80-amino-acid motif, the SRY-box, which shares a high degree of homology with a DNA-binding domain found in the high-mobility-group (HMG) proteins HMG1 and HMG2. The SRY box motif is highly conserved in several sequence-specific DNA-binding proteins that are known to act as transcription factors. Here we describe the use of degenerate PCR primers to identify SRY-related sequences containing the SRY-box motif from the genomic DNA of a variety of species. The results of this study suggest that in a diverse array of species SRY-related genes may serve as transcription factors that regulate a variety of developmental pathways, including sex determination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alligators and Crocodiles
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Evolution*
  • Chickens
  • DNA
  • DNA-Binding Proteins / genetics*
  • Drosophila
  • Humans
  • Lizards
  • Molecular Sequence Data
  • Nuclear Proteins*
  • Polymerase Chain Reaction*
  • Sequence Homology, Amino Acid
  • Sex-Determining Region Y Protein
  • Transcription Factors*


  • DNA-Binding Proteins
  • Nuclear Proteins
  • SRY protein, human
  • Sex-Determining Region Y Protein
  • Transcription Factors
  • DNA

Associated data

  • GENBANK/M86310
  • GENBANK/M86311
  • GENBANK/M86312
  • GENBANK/M86313
  • GENBANK/M86314
  • GENBANK/M86315
  • GENBANK/M86316
  • GENBANK/M86317
  • GENBANK/M86318
  • GENBANK/M86319
  • GENBANK/M86320
  • GENBANK/M86321
  • GENBANK/M86322
  • GENBANK/M86323
  • GENBANK/M86324
  • GENBANK/M86325
  • GENBANK/M86326
  • GENBANK/M86327
  • GENBANK/M86328
  • GENBANK/M86329
  • GENBANK/M86330
  • GENBANK/M86331
  • GENBANK/M86332
  • GENBANK/M86333
  • GENBANK/M86334
  • GENBANK/M86335
  • GENBANK/M86336
  • GENBANK/M86337
  • GENBANK/M86338
  • GENBANK/M86339