Detection of Aeromonas hydrophila in food with an enzyme-linked immunosorbent assay

J Appl Bacteriol. 1993 Feb;74(2):149-54. doi: 10.1111/j.1365-2672.1993.tb03008.x.

Abstract

A microtitration plate, antibody capture, enzyme-linked immunosorbent assay was developed for the detection of Aeromonas hydrophila serotype O: 11 (highly virulent strains). The assay utilizes a detector antibody which shows no cross-reactions with Aeromonas strains other than serotype O: 11 or non-Aeromonas competing organisms. The detector antibody is mixed with the sample and incubated for 1 h, microcentrifuged and the supernatant fluid (unadsorbed antibody) titred in a microtitre plate coated with A. hydrophila cells from serotype O: 11. All the A. hydrophila strains from serotype O: 11 tested reacted strongly with the detector antibody. Also by culturing and performing the immunoassay with the detector antibody we established and quantified the presence of A. hydrophila O: 11 in different foods.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aeromonas hydrophila / immunology
  • Aeromonas hydrophila / isolation & purification*
  • Antibodies, Bacterial / immunology
  • Antibody Specificity
  • Bacterial Outer Membrane Proteins / analysis
  • Bacterial Outer Membrane Proteins / immunology
  • Bacterial Proteins*
  • Blotting, Western
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Food Microbiology*
  • Membrane Glycoproteins / analysis
  • Membrane Glycoproteins / immunology
  • Sensitivity and Specificity

Substances

  • Antibodies, Bacterial
  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Membrane Glycoproteins
  • S-layer proteins