The Escherichia coli FtsH protein is a prokaryotic member of a protein family of putative ATPases involved in membrane functions, cell cycle control, and gene expression

J Bacteriol. 1993 Mar;175(5):1344-51. doi: 10.1128/jb.175.5.1344-1351.1993.


The ftsH gene is essential for cell viability in Escherichia coli. We cloned and sequenced the wild-type ftsH gene and the temperature-sensitive ftsH1(Ts) gene. It was suggested that FtsH protein was an integral membrane protein of 70.7 kDa (644 amino acid residues) with a putative ATP-binding domain. The ftsH1(Ts) gene was found to have two base substitutions within the coding sequence corresponding to the amino acid substitutions Glu-463 by Lys and Pro-587 by Ala. Homology search revealed that an approximately 200-amino-acid domain, including the putative ATP-binding sequence, is highly homologous (35 to 48% identical) to the domain found in members of a novel, eukaryotic family of putative ATPases, e.g., Sec18p, Pas1p, CDC48p, and TBP-1, which function in protein transport pathways, peroxisome assembly, cell division cycle, and gene expression, respectively. Possible implications of these observations are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Dependent Proteases
  • Adenosine Triphosphatases / genetics
  • Adenosine Triphosphatases / metabolism*
  • Amino Acid Sequence
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Base Sequence
  • Cell Cycle / physiology
  • Cell Membrane / physiology
  • Cloning, Molecular
  • DNA, Bacterial
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics
  • Escherichia coli Proteins
  • Gene Expression Regulation, Bacterial / physiology
  • Genes, Bacterial
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Molecular Sequence Data
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics
  • Sequence Homology, Amino Acid


  • Bacterial Proteins
  • DNA, Bacterial
  • Escherichia coli Proteins
  • Membrane Proteins
  • ATP-Dependent Proteases
  • FtsH protein, E coli
  • Adenosine Triphosphatases

Associated data

  • GENBANK/M83138