SPL1-1, a Saccharomyces cerevisiae mutation affecting tRNA splicing

J Bacteriol. 1993 Mar;175(5):1433-42. doi: 10.1128/jb.175.5.1433-1442.1993.

Abstract

A genetic approach was used to isolate and characterize Saccharomyces cerevisiae genes affecting tRNA processing. Three mutants were isolated which were able to process and utilize splicing-deficient transcripts from inactivated Schizosaccharomyces pombe suppressor tRNA genes. Extragenic recovery of suppressibility was verified by the suppression of nonsense mutations in LEU2, HIS4, and ADE1. One mutant, SPL1-1, was chosen for detailed analysis on the basis of its increased synthesis of mature suppressor tRNA over wild-type cell levels as determined by Northern (RNA) analysis. This mutant exhibited strong suppression exclusively with the defective tRNA gene used in the mutant selection. Genetic analysis revealed that a single, dominant, haplo-lethal mutation was responsible for the suppression phenotype. The mutation mapped on chromosome III to an essential 1.5-kb open reading frame (L. S. Symington and T. D. Petes, Mol. Cell. Biol. 8:595-604, 1988), recently named NFS1 (S. G. Oliver et al., Nature [London] 357:38-46, 1992), located adjacent (centromere proximal) to LEU2.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Introns
  • Molecular Sequence Data
  • Mutation*
  • Nucleic Acid Conformation
  • RNA Processing, Post-Transcriptional
  • RNA Splicing / genetics*
  • RNA, Fungal / chemistry
  • RNA, Fungal / genetics
  • RNA, Transfer / chemistry
  • RNA, Transfer / genetics*
  • Saccharomyces cerevisiae / genetics*
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic

Substances

  • RNA, Fungal
  • RNA, Transfer

Associated data

  • GENBANK/M98808
  • GENBANK/X05459
  • SWISSPROT/P25374