The structure of the 5' terminal cap of the respiratory syncytial virus mRNA

J Gen Virol. 1993 Mar;74 ( Pt 3):485-90. doi: 10.1099/0022-1317-74-3-485.

Abstract

The 5' terminal cap structure of the mRNAs of human respiratory syncytial virus synthesized in vitro in the presence of S-adenosyl-L-methionine consists of a 7-methyl guanosine linked to an unmethylated guanosine through a 5'-5' pyrophosphate linkage formed by using the alpha and beta phosphates of GTP. The complete cap structure is m7G (5')ppp(5')Gp ... which is devoid of ribose 2'-O-methylation. Capping, including methylation, is coupled to transcription. These results constitute the first report of a pneumoviral mRNA cap structure.

MeSH terms

  • Base Sequence
  • Molecular Sequence Data
  • RNA Caps / genetics*
  • RNA, Messenger / genetics*
  • RNA, Viral / genetics*
  • Respiratory Syncytial Viruses / genetics*

Substances

  • RNA Caps
  • RNA, Messenger
  • RNA, Viral