A new component of the transcription factor DRTF1/E2F

Nature. 1993 Mar 4;362(6415):83-7. doi: 10.1038/362083a0.


Transcription factor DRTF1/E2F coordinates events in the cell cycle with transcription by its cyclical interactions with important regulators of cellular proliferation like the retinoblastoma tumour-suppressor gene product (Rb) and the Rb-related protein, p107 (refs 1-8). DRTF1/E2F binding sites occur in the control regions of genes involved in proliferation, and both Rb and p107 repress the capacity of DRTF1/E2F to activate transcription (refs 11, 12; M. Zamanian and N.B.L.T., manuscript submitted). Mutant Rb proteins isolated from tumour cells are unable to bind DRTF1/E2F (refs 11-13), and certain viral oncoproteins, such as adenovirus E1A, sequester Rb and p107 in order to free active DRTF1/E2F (refs 5, 11, 12, 14, 15). Here we report the isolation of a complementary DNA encoding DRTF1-polypeptide-1 (DP-1), a major sequence-specific binding protein that is present in DRTF1/E2F, including Rb- and p107-associated DRTF1/E2F. The DNA-binding domain of DP-1 contains a region that resembles that of E2F-1 (refs 16, 17), and recognizes the same sequence. DRTF1/E2F thus appears to contain at least two sequence-specific DNA-binding proteins.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenovirus E2 Proteins / genetics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carrier Proteins*
  • Cell Cycle Proteins*
  • DNA-Binding Proteins*
  • E2F Transcription Factors
  • E2F1 Transcription Factor
  • HeLa Cells
  • Humans
  • Mice
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Oligonucleotides, Antisense
  • Polymerase Chain Reaction / methods
  • Promoter Regions, Genetic
  • Retinoblastoma-Binding Protein 1
  • Sequence Homology, Amino Acid
  • Teratoma
  • Transcription Factor DP1
  • Transcription Factors / genetics*
  • Transcription Factors / isolation & purification
  • Transcription Factors / metabolism
  • Tumor Cells, Cultured


  • Adenovirus E2 Proteins
  • Arid4a protein, mouse
  • Carrier Proteins
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • E2F Transcription Factors
  • E2F1 Transcription Factor
  • E2F1 protein, human
  • E2f1 protein, mouse
  • Oligodeoxyribonucleotides
  • Oligonucleotides, Antisense
  • Retinoblastoma-Binding Protein 1
  • TFDP1 protein, human
  • Tfdp1 protein, mouse
  • Transcription Factor DP1
  • Transcription Factors

Associated data

  • GENBANK/X72310