The aim of this study was to assess the internight and intrasubject variability of nocturnal activity and immobility measures of 99 healthy subjects aged 50-98 years. Motor activity was recorded at home during 6 successive nights with a wrist-worn activity monitor. The occurrence of suprathreshold motor activity was recorded over 15-second epochs. For each subject, six mean measures reflecting activity or immobility during sleep and their coefficient of variation were calculated. Our results revealed no first-night effect or day-of-week effect of the activity and immobility measures over the 6 nights across all subjects. On the other hand, for all nocturnal activity and immobility measures, a considerable intrasubject variability across the 6 nights was found. Females had a greater intrasubject variability of the mean duration of immobility periods and the movement index than males. The intrasubject variability of all nocturnal activity and immobility measures across the successive age groups remains stable. These findings emphasize that although a first-night effect may be lacking, the intrasubject variability of activity and immobility measures across several nights may still be considerable.