Determinants of protein modification versus heme alkylation: inactivation of cytochrome P450 1A1 by 1-ethynylpyrene and phenylacetylene

Chem Res Toxicol. 1993 Jan-Feb;6(1):38-45. doi: 10.1021/tx00031a006.


Reaction of cytochrome P450 enzymes with arylacetylenes results in heme N-alkylation [e.g., Komives, E. A., and Ortiz de Montellano, P. R., (1985) J. Biol. Chem. 260, 3330-3336] and/or protein modification [e.g., Gan, L.-S. L., Acebo, A. L. and Alworth, W. L. (1984) Biochemistry 23, 3827-3836]. To clarify the factors that determine whether heme or protein alkylation occurs, we have investigated the cytochrome P450 1A1-catalyzed oxidation of 1-ethynylpyrene (1-EP) and phenylacetylene (PA). Cytochrome P450 1A1 in microsomes from beta-naphthoflavone-induced rats is inactivated in a time- and NADPH-dependent manner by 1-EP and PA. Parallel loss of the heme chromophore is observed with PA but not with 1-EP, although partial heme chromophore loss is observed when the purified, reconstituted enzyme is inactivated by either agent. Product analysis shows that 1-EP and PA are oxidized to, respectively, (1'-pyrenyl)-acetic and phenylacetic acids. In contrast to the inactivation of cytochrome P450 2B1 by PA, no isotope effect is observed on enzyme inactivation or metabolite formation when the acetylenic hydrogen is replaced by deuterium in either 1-EP or PA. Inactivation of cytochrome P450 1A1 by 1-EP results in covalent binding of 0.8-0.9 equiv (relative to total cytochrome P450 content) of the inhibitor to the microsomal protein. The results demonstrate that a single isozyme can be inactivated, depending on the structure of the arylacetylene, by heme or protein alkylation. Spectroscopic binding constants (Ks) show that 1-EP binds to the enzyme with > 2000 times greater affinity that PA.(ABSTRACT TRUNCATED AT 250 WORDS)

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetylene / analogs & derivatives*
  • Acetylene / pharmacology
  • Alkylation
  • Animals
  • Benzopyrene Hydroxylase / antagonists & inhibitors*
  • Cytochrome P-450 CYP1A1
  • Cytochrome P-450 Enzyme Inhibitors*
  • Cytochrome P-450 Enzyme System / isolation & purification
  • Cytochrome P-450 Enzyme System / metabolism
  • Heme / chemistry*
  • In Vitro Techniques
  • Isoenzymes / antagonists & inhibitors*
  • Isoenzymes / isolation & purification
  • Male
  • Microsomes, Liver / drug effects
  • Microsomes, Liver / enzymology
  • NADP / metabolism
  • Oxidation-Reduction
  • Oxidoreductases / metabolism
  • Proteins / chemistry
  • Proteins / drug effects*
  • Pyrenes / pharmacology*
  • Rats
  • Rats, Sprague-Dawley


  • Cytochrome P-450 Enzyme Inhibitors
  • Isoenzymes
  • Proteins
  • Pyrenes
  • phenylacetylene
  • 1-ethynylpyrene
  • Heme
  • NADP
  • Cytochrome P-450 Enzyme System
  • Oxidoreductases
  • Benzopyrene Hydroxylase
  • Cytochrome P-450 CYP1A1
  • Acetylene