Cloning, analysis, and chromosomal localization of Notch-1, a mouse homolog of Drosophila Notch

Genomics. 1993 Feb;15(2):259-64. doi: 10.1006/geno.1993.1055.


The Notch gene of Drosophila encodes a large transmembrane protein involved in cell-cell interactions and cell fate decisions in the Drosophila embryo. We report here the isolation of cDNA clones encompassing the full-length coding sequence of Notch-1, a mouse homolog of Drosophila Notch. The predicted amino acid sequence of the Notch-1 protein retains all of the conserved amino acid motifs of Notch and the other vertebrate Notch homologs. The cDNA sequence predicts a 2531-amino-acid protein containing a signal peptide, 36 epidermal growth factor-like repeats, 3 Notch/lin-12 repeats, a transmembrane domain, and 6 cdc10/ankyrin repeats. The Notch-1 gene was localized to the proximal portion of mouse chromosome 2 by mapping with an interspecific backcross panel.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromosome Mapping*
  • Cloning, Molecular
  • DNA
  • Drosophila
  • Drosophila Proteins
  • Female
  • Insect Hormones / genetics*
  • Male
  • Membrane Proteins / genetics*
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Receptor, Notch1
  • Receptors, Cell Surface*
  • Receptors, Notch
  • Sequence Homology, Amino Acid
  • Transcription Factors*


  • Drosophila Proteins
  • Insect Hormones
  • Membrane Proteins
  • N protein, Drosophila
  • Notch1 protein, mouse
  • Receptor, Notch1
  • Receptors, Cell Surface
  • Receptors, Notch
  • Transcription Factors
  • DNA

Associated data

  • GENBANK/L14299
  • GENBANK/L14300
  • GENBANK/L14301
  • GENBANK/U04288
  • GENBANK/U04289
  • GENBANK/U04290
  • GENBANK/U04291
  • GENBANK/U04292
  • GENBANK/U04293
  • GENBANK/Z11886