Abnormal peptidoglycan produced in a methicillin-resistant strain of Staphylococcus aureus grown in the presence of methicillin: functional role for penicillin-binding protein 2A in cell wall synthesis

Antimicrob Agents Chemother. 1993 Feb;37(2):342-6. doi: 10.1128/AAC.37.2.342.

Abstract

Upon the addition of methicillin even at relatively low concentrations (5 mug/ml or 0.3% of the MIC) to the medium, methicillin-resistant staphylococci shift to the production of a new peptidoglycan with an abnormal muropeptide composition which may be the synthetic product of penicillin-binding protein 2A.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins*
  • Carrier Proteins / physiology*
  • Cell Wall / drug effects
  • Cell Wall / metabolism*
  • Cell Wall / ultrastructure
  • Chromatography, High Pressure Liquid
  • Hexosyltransferases*
  • Methicillin / pharmacology*
  • Methicillin Resistance / physiology*
  • Microscopy, Electron
  • Molecular Sequence Data
  • Muramoylpentapeptide Carboxypeptidase / physiology*
  • Penicillin-Binding Proteins
  • Peptidoglycan / analysis
  • Peptidoglycan / biosynthesis*
  • Peptidyl Transferases*
  • Staphylococcus aureus / metabolism*
  • Staphylococcus aureus / ultrastructure
  • Substrate Specificity

Substances

  • Bacterial Proteins
  • Carrier Proteins
  • Penicillin-Binding Proteins
  • Peptidoglycan
  • Peptidyl Transferases
  • Hexosyltransferases
  • Muramoylpentapeptide Carboxypeptidase
  • Methicillin