Isolation of a cDNA encoding the human brain serotonin transporter

J Neural Transm Gen Sect. 1993;91(1):67-72. doi: 10.1007/BF01244919.


A cDNA encoding a serotonin transporter (5-HTT) in the human dorsal raphe nucleus was isolated and sequenced using cross-species amplification of human 5-HTT partial cDNA by the polymerase chain reaction (PCR) and the RACE-PCR procedure, designed for rapid amplification of 3' and 5' cDNA ends. The cDNA contains an open reading frame encoding a hydrophobic polypeptide of 630 amino acids with a calculated molecular weight of approximately 70 kDa. The human 5-HTT is approximately 92% homologous to the rat protein but contains an additional consensus phosphorylation site for cAMP-dependent protein kinase recognition located in the cytoplasmic N-terminal region, while a potential protein kinase C phosphorylation site identified in the rat homolog is not conserved in the human 5-HTT. Hydropathicity analysis revealed twelve membrane spanning segments, a topology proposed for other cloned sodium-dependent transporters.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Carrier Proteins / genetics*
  • Consensus Sequence
  • DNA / genetics*
  • Membrane Glycoproteins / genetics*
  • Membrane Transport Proteins*
  • Molecular Sequence Data
  • Molecular Weight
  • Nerve Tissue Proteins / genetics*
  • Open Reading Frames
  • Phosphorylation
  • Polymerase Chain Reaction
  • Protein Kinase C / metabolism
  • Protein Kinases / metabolism
  • Protein Processing, Post-Translational
  • Raphe Nuclei / chemistry*
  • Rats
  • Serotonin / metabolism*
  • Serotonin Plasma Membrane Transport Proteins


  • Carrier Proteins
  • Membrane Glycoproteins
  • Membrane Transport Proteins
  • Nerve Tissue Proteins
  • Serotonin Plasma Membrane Transport Proteins
  • Slc6a4 protein, rat
  • Serotonin
  • DNA
  • Protein Kinases
  • Protein Kinase C

Associated data

  • GENBANK/X70697