Rainbow trout ovarian cholesterol side-chain cleavage cytochrome P450 (P450scc). cDNA cloning and mRNA expression during oogenesis

FEBS Lett. 1993 Mar 15;319(1-2):45-8. doi: 10.1016/0014-5793(93)80034-r.


A cDNA clone encoding cholesterol side-chain cleavage cytochrome P450 (P450scc) was isolated from a rainbow trout ovarian follicle cDNA library. The cDNA contains an open reading frame of 1,542 nucleotides encoding a protein of 514 amino acids. The predicted amino acid sequence of trout P450scc shows 48% homology with that of human, and 46% homology with that of rat, bovine and pig. P450scc activity was confirmed by transfected COS-1 monkey kidney tumour cells with an expression vector for trout P450scc cDNA and subsequent detection of conversion from 25-hydroxycholesterol to pregnenolone by radioimmunoassay. The cDNA only hybridized to a single 1.8 kb RNA transcript. The transcript was not found in early vitellogenic follicles, barely detected in postvitellogenic follicles, and abundant in postovulatory follicles.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cholesterol Side-Chain Cleavage Enzyme / genetics*
  • Cloning, Molecular
  • Codon
  • DNA / chemistry
  • DNA / genetics*
  • DNA / isolation & purification
  • Female
  • Gene Expression*
  • Humans
  • Molecular Sequence Data
  • Oogenesis / genetics*
  • Ovary / enzymology*
  • RNA, Messenger / metabolism
  • Sequence Homology, Amino Acid
  • Transfection
  • Trout / genetics*


  • Codon
  • RNA, Messenger
  • DNA
  • Cholesterol Side-Chain Cleavage Enzyme