Chicken anti-protein G for the detection of small amounts of protein G

Hybridoma. 1993 Feb;12(1):143-7. doi: 10.1089/hyb.1993.12.143.

Abstract

Streptococcal protein G, similar to staphylococcal protein A, is the Fc-binding proteins that seems best suited for affinity-purification of mouse, rat or human monoclonal antibodies. We have developed a sandwich-ELISA for monitoring of protein G leakage from such columns. It utilizes microtitre plates coated with chicken anti-protein G to capture protein G, and biotinylated chicken anti-protein G to detect bound protein G. Chicken IgG is one of a very few IgG that does not show any Fc reactivity with protein G. It is thus possible to make an antigen-specific assay for protein G. This assay can be used to detect protein G in IgG containing solutions, such as monoclonal antibody preparations. The method can be used to detect 1 x 10(-7) g protein G/1 in the presence of human serum or human IgG.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / analysis
  • Bacterial Proteins / immunology*
  • Chickens
  • Chromatography, Affinity
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Mice
  • Rats

Substances

  • Bacterial Proteins
  • IgG Fc-binding protein, Streptococcus