Sequence analysis and antigenic cross-reactivity of a venom allergen, antigen 5, from hornets, wasps, and yellow jackets

J Immunol. 1993 Apr 1;150(7):2823-30.


Ag 5 is a major allergen of vespid venom. The complete amino acid sequences of Ag 5 from two species each of hornets (Dolichovespula arenaria and maculata), wasps (Polistes annularis and exclamans), and yellow jackets (Vespula maculifrons and vulgaris) have been determined by amino acid sequencing and/or cDNA cloning. The sequence data reveal several conserved and variable regions for vespid Ag 5. The conserved regions show greater sequence similarity than do the less conserved regions to a family of proteins from human, mouse, and rat testis and to a class of pathogenesis-related proteins from tobacco and tomato leaves. Tests in the murine system for both T and B cell responses demonstrate a high degree of cross-reactivity of hornet Ag 5 with wasp or yellow jacket Ag 5 and a low degree of cross-reactivity of yellow jacket Ag 5 with wasp Ag 5. This pattern of cross-reactivity is in the same order as their sequence similarity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Allergens / chemistry*
  • Allergens / genetics
  • Allergens / immunology*
  • Amino Acid Sequence
  • Animals
  • B-Lymphocytes / immunology
  • Base Sequence
  • Cross Reactions
  • Female
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • T-Lymphocytes / immunology
  • Wasp Venoms / genetics
  • Wasp Venoms / immunology*
  • Wasp Venoms / isolation & purification*
  • Wasps / immunology


  • Allergens
  • Wasp Venoms
  • white-faced hornet venom antigen 5

Associated data

  • GENBANK/M98857
  • GENBANK/M98858
  • GENBANK/M98859