Identification of an essential Drosophila gene that is homologous to the translation initiation factor eIF-4A of yeast and mouse

Mol Gen Genet. 1993 Feb;237(1-2):233-40. doi: 10.1007/BF00282805.


A gene encoding a protein homologous to the translation initiation factor eIF-4A in mouse has been identified in Drosophila melanogaster. The predicted amino acid sequence shows 73% identity with the mouse gene and 67% identity with a homologous protein from yeast. The single-copy Drosophila gene is located on chromosome arm 2L at 26A7-9. Several recessive lethal mutations have been isolated and genetically characterized. Northern blot hybridization shows two abundant transcripts of 1.75 kb and 1.9 kb throughout all developmental stages. Both transcripts are maternally provided to the oocyte.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Crosses, Genetic
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics*
  • Eukaryotic Initiation Factor-4A
  • Genes, Insect / genetics*
  • Genes, Lethal / genetics*
  • Genetic Complementation Test
  • Mice / genetics
  • Molecular Sequence Data
  • Peptide Initiation Factors / genetics*
  • Protein Biosynthesis / genetics*
  • RNA, Messenger / genetics
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics
  • Sequence Homology, Amino Acid


  • Peptide Initiation Factors
  • RNA, Messenger
  • Eukaryotic Initiation Factor-4A

Associated data

  • GENBANK/X69045