Ca2+ binding of latent transforming growth factor-beta 1 binding protein

FEBS Lett. 1993 Apr 5;320(2):140-4. doi: 10.1016/0014-5793(93)80079-a.


Latent transforming growth factor-beta 1 binding protein (LTBP) is a constituent of the latent high molecular weight complex of TGF-beta 1 in human platelets. In the present communication, we show that LTBP binds Ca2+ in its free form as well as in the latent TGF-beta 1 complex. The binding of Ca2+ induces a structural change which protects the molecule against proteolysis and changes its elution position when analyzed by anion exchange chromatography. The in vitro activation of TGF-beta 1 is not influenced by the presence of Ca2+. The possible significance of Ca2+ binding of LTBP is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium / metabolism*
  • Carrier Proteins / chemistry
  • Carrier Proteins / metabolism*
  • Cell Line
  • Humans
  • Intracellular Signaling Peptides and Proteins*
  • Latent TGF-beta Binding Proteins
  • Protein Conformation
  • Recombinant Proteins / metabolism
  • Transforming Growth Factor beta / metabolism*


  • Carrier Proteins
  • Intracellular Signaling Peptides and Proteins
  • Latent TGF-beta Binding Proteins
  • Recombinant Proteins
  • Transforming Growth Factor beta
  • Calcium