Angiogenic activity of peritoneal fluid from women with endometriosis

Fertil Steril. 1993 Apr;59(4):778-82. doi: 10.1016/s0015-0282(16)55859-2.


Objective: To investigate the presence of angiogenic factors in peritoneal fluid (PF) and follicular fluid (FF).

Design: The PF samples of 48 women with (n = 24) or without (n = 24) endometriosis were investigated. Angiogenesis was assayed using the chorioallantoic membrane of 11-day-old fertilized chicken embryos. Glass fiber prefilters impregnated with the fluids were placed on the chorioallantoic membrane.

Main outcome measure: The vascular reaction was analyzed by an independent observer after 72 hours.

Results: There was a positive reaction in 58.3% of the PF from women with endometriosis and in 12.5% of the women without endometriosis. No correlation was found between the angiogenic response and the severity of endometriosis. The reaction remained after charcoal treatment of the PF. Positive reaction was found in three of six FF samples.

Conclusion: The PF of women with endometriosis contain more angiogenic factors than PF from women without endometriosis. This angiogenic activity could be important for the further outgrowth and progression of the lesions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiogenesis Inducing Agents / analysis*
  • Animals
  • Ascitic Fluid / chemistry*
  • Biological Assay
  • Chick Embryo
  • Endometriosis / etiology
  • Endometriosis / immunology
  • Endometriosis / metabolism*
  • Female
  • Humans
  • Killer Cells, Natural / immunology


  • Angiogenesis Inducing Agents