Molecular cloning of a rho family gene of Entamoeba histolytica

Mol Biochem Parasitol. 1993 Mar;58(1):177-80. doi: 10.1016/0166-6851(93)90104-6.

Abstract

An Entamoeba histolytica gene (Eh rho1) was cloned that encodes a putative low-molecular-mass GTP-binding protein, most similar to the ras homologue rho. The Eh rho1 open reading frame was 208 amino acids long and encoded a 23-kDa protein similar to Saccharomyces cerevisiae RHO1-RHO4 and CDC42 and human rhoA, rac1, and G25K gene products. This similarity was greatest at the NH2 terminus of Eh rho1 where two GTP-binding sites and a possible effector site were conserved. A cysteine residue at the COOH terminus of Eh rho1 was followed by eight hydrophobic amino acids rather than the three hydrophobic amino acids present in other ras family proteins.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Entamoeba histolytica / genetics*
  • Fungal Proteins / genetics*
  • GTP-Binding Proteins / genetics*
  • Genes, Protozoan / genetics*
  • Genes, ras / genetics
  • Molecular Sequence Data
  • Multigene Family / genetics
  • Open Reading Frames
  • Phylogeny
  • Sequence Homology, Amino Acid
  • rho GTP-Binding Proteins*

Substances

  • Fungal Proteins
  • GTP-Binding Proteins
  • rho GTP-Binding Proteins

Associated data

  • GENBANK/L03809