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Comparative Study
. 1993 Apr;193(2):981-5.
doi: 10.1006/viro.1993.1210.

Identification of a Region in the Pr55gag-polyprotein Essential for HIV-1 Particle Formation

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Comparative Study

Identification of a Region in the Pr55gag-polyprotein Essential for HIV-1 Particle Formation

A von Poblotzki et al. Virology. .

Abstract

The pr55gag polyprotein of HIV-1 plays a critical role in the formation of immature virus particles in the cell and during the budding process. We investigated the influence of amino acid substitutions in the p24CA- region of the gag polyprotein on the viral assembly process. Deletion of the amino acids 341-352 in the carboxy terminal part of the p24CA resulted in a loss of the capacity of the gag polyprotein to form virus-like particles when expressed in eucaryotic cells by recombinant vaccinia virus. In further experiments it turned out that the amino acids 341-346 and 350-352 are important for the ability of the pr55gag to form virus-like particles. Because these stretches are conserved among HIV-1, HIV-2, SIV, and FIV, we conclude that these amino acids form a domain highly important for the assembly of these lentiviruses.

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