Erythroid Transcription Factor GATA-1 Is Abundantly Transcribed in Mouse Testis

Nature. 1993 Apr 1;362(6419):466-8. doi: 10.1038/362466a0.

Abstract

The transcription factor GATA-1 is a fundamental regulator of genes in haematopoietic cell lineages and belongs to a family of factors that bind to the consensus sequence WGATAR. The GATA motif was originally identified in cis-regulatory regions of globin and other erythroid-specific genes, but the range of genes controlled by the GATA factors has since expanded. Members of the GATA transcription factor family share a conserved zinc-finger DNA-binding domain, but the expression profile of each GATA factor is distinct. Here we show that a testis form of murine (m)GATA-1 messenger RNA is transcribed from a promoter located 5' to the erythroid first exon, and the remaining exons (which encode the mGATA-1 protein) are used in common by both testis and erythroid transcripts. We use an anti-mGATA-1 monoclonal antibody to show that the factor expressed in erythroid cells is the same as that found in the seminiferous tubules of murine testis. The GATA-1-expressing cells in 10-week-old testis were found only in contact with the basement membrane of seminiferous tubules, suggesting that GATA-1 regulates genes during the earliest stages of spermatogenesis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aging / physiology
  • Animals
  • Base Sequence
  • DNA-Binding Proteins / genetics*
  • Erythroid-Specific DNA-Binding Factors
  • GATA1 Transcription Factor
  • Gene Expression
  • Gene Library
  • Male
  • Mice
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Organ Specificity
  • Polymerase Chain Reaction / methods
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Homology, Nucleic Acid
  • Testis / cytology
  • Testis / growth & development
  • Testis / physiology*
  • Transcription Factors / genetics*
  • Transcription, Genetic*
  • Zinc Fingers / genetics

Substances

  • DNA-Binding Proteins
  • Erythroid-Specific DNA-Binding Factors
  • GATA1 Transcription Factor
  • Gata1 protein, mouse
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Transcription Factors