Studying isolation technique with DEAE amylum the method was found to be suitable for isolation of alpha-amylase. After determination of optimal conditions sorption curves were illustrated, from which the amount of DEAE amylum needed for 100% enzyme sorption from solutions of various activity and origin can be calculated. Preparation obtained shows high temperature stability and can be used in food industry and agriculture or after its elution in pharmacy. At the end of the paper laboratory isolation technique is described.