Strand-specific FISH reveals orientation of chromosome 18 alphoid DNA

Cytogenet Cell Genet. 1993;63(2):126-7. doi: 10.1159/000133516.


A method is described for making in situ hybridization strand specific. Through the use of a synthetic DNA probe of a repetitive sequence in the centromeric region of chromosome 18, it is shown that the repeats exist in a head-to-tail tandem array. The method should be useful for studies of the molecular organization and mapping of chromosomes.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence
  • Cell Line
  • Chromosomes, Human, Pair 18*
  • DNA / genetics
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Molecular Sequence Data
  • Repetitive Sequences, Nucleic Acid*


  • DNA