The control in cis of the position and the amount of the ARG4 meiotic double-strand break of Saccharomyces cerevisiae

EMBO J. 1993 Apr;12(4):1459-66.


During meiosis, a transient DNA double-strand break (DSB) occurs in the promoter region (positions -200/-185) of the Saccharomyces cerevisiae ARG4 gene and is a likely intermediate in the initiation of meiotic gene conversion events in this region. We report here a functional analysis of the ARG4 DSB based on the study of various deletions in this chromosomal region. We have identified several cis-acting elements located within the -465/+3 region of the ARG4 promoter that control the formation of this DSB. The -465/-317 region includes a transcription terminator and is necessary for a normal amount of ARG4 DSB, but not for its positioning. The -316/-140 region can be replaced by an unrelated DNA sequence where a meiotic DSB then occurs, suggesting that the site of DSB is not sequence-specific, but is positioned at a fixed distance from the adjacent -139/+3 region. Also, in all strains constructed, the amount of meiotic DSB is correlated with the frequency of gene conversion in ARG4, which provides a strong argument for the initiation of gene conversion by a DSB in this region of the yeast genome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Mutational Analysis
  • DNA, Fungal / genetics
  • Fungal Proteins / genetics
  • Gene Conversion*
  • Genes, Fungal*
  • Meiosis*
  • Promoter Regions, Genetic
  • Saccharomyces cerevisiae / genetics*
  • Sequence Deletion


  • DNA, Fungal
  • Fungal Proteins