Isolation and characterization of the mouse thrombospondin 3 (Thbs3) gene

Genomics. 1993 Mar;15(3):607-13. doi: 10.1006/geno.1993.1114.


The DNA sequence of the coding region of the mouse thrombospondin (TSP) 3 gene has been determined by analysis of both genomic and cDNA clones. Like TSP1 and TSP2, TSP3 has a homologous COOH-terminal domain and seven type III (Ca(2+)-binding) repeats. However, TSP3 contains four, rather than three, type II repeats and lacks the type I (TSP or properdin) repeats and procollagen homology characteristic of TSP1 and TSP2. In addition, the NH2-terminal domain of TSP3 differs markedly, both in sequence and in exon/intron structure, from that in TSP1 or TSP2. The gene is located on mouse chromosome 3, bands E3-F1, immediately upstream from the Muc1 (episialin) gene and is expressed in the developing mouse in a pattern that also differs from that of TSP1 or TSP2. Based on its structure, we suggest that TSP3 may play both a unique role in cell-matrix interactions and perform functions that overlap with those of TSP1 and TSP2.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Adhesion Molecules / genetics*
  • Chromosome Mapping
  • DNA
  • Exons
  • Introns
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Platelet Membrane Glycoproteins / genetics*
  • Sequence Homology, Nucleic Acid
  • Thrombospondins


  • Cell Adhesion Molecules
  • Platelet Membrane Glycoproteins
  • Thrombospondins
  • DNA

Associated data

  • GENBANK/L04302
  • GENBANK/S58733
  • GENBANK/S58735
  • GENBANK/U04288
  • GENBANK/U04289
  • GENBANK/U04290
  • GENBANK/U04291
  • GENBANK/U04292
  • GENBANK/U04293
  • GENBANK/X73110