Production of an interleukin-8-like chemokine by cytokine-stimulated rat NRK-49F fibroblasts and its suppression by anti-inflammatory steroids

Biochem Pharmacol. 1993 Apr 6;45(7):1425-30. doi: 10.1016/0006-2952(93)90041-t.


Normal rat kidney fibroblasts (NRK-49F cells) stimulated with interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) produced mainly cytokine-induced neutrophil chemoattractant (CINC) which is the rat counterpart of human gro/melanoma growth stimulatory activity. In addition, the cytokine-stimulated cells produced two minor neutrophil chemoattractants which are highly related to murine macrophage inflammatory protein-2 in their NH2-terminal amino acid sequences. IL-1 beta was a stronger stimulator than TNF-alpha, and addition of both the cytokines into the NRK-49F cell culture caused an additive stimulation for rat gro/CINC production. The anti-inflammatory steroids (dexamethasone, prednisolone and hydrocortisone) at 10(-9)-10(-6) M significantly suppressed the production of rat gro/CINC by the IL-1 beta-stimulated NRK-49F cells in a dose-dependent manner. The relative potencies of the inhibitory activity of the steroids on the rat gro/CINC production were dexamethasone > prednisolone > hydrocortisone. On the other hand, the non-steroidal anti-inflammatory drugs (indomethacin and piroxicam) at 10(-7)-10(-5) M showed no apparent inhibitory effect on rat gro/CINC production by NRK-49F cells stimulated with IL-1 beta.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Cell Line / drug effects
  • Chemokine CXCL1
  • Chemokine CXCL2
  • Chemokines, CXC*
  • Chemotactic Factors / biosynthesis*
  • Fibroblasts / drug effects
  • Growth Substances / biosynthesis*
  • Intercellular Signaling Peptides and Proteins*
  • Interleukin-1 / antagonists & inhibitors
  • Interleukin-1 / pharmacology*
  • Interleukin-8 / biosynthesis*
  • Molecular Sequence Data
  • Rats
  • Recombinant Proteins / pharmacology
  • Steroids
  • Tumor Necrosis Factor-alpha / antagonists & inhibitors
  • Tumor Necrosis Factor-alpha / pharmacology*


  • Anti-Inflammatory Agents
  • CXCL1 protein, human
  • Chemokine CXCL1
  • Chemokine CXCL2
  • Chemokines, CXC
  • Chemotactic Factors
  • Cxcl1 protein, rat
  • Cxcl2 protein, rat
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Interleukin-1
  • Interleukin-8
  • Recombinant Proteins
  • Steroids
  • Tumor Necrosis Factor-alpha