Delayed effects of neonatal exposure to Tween 80 on female reproductive organs in rats

Food Chem Toxicol. 1993 Mar;31(3):183-90. doi: 10.1016/0278-6915(93)90092-d.


Neonatal female rats were injected ip (0.1 ml/rat) with Tween 80 in 1, 5 or 10% aqueous solution on days 4-7 after birth. Treatment with Tween 80 accelerated maturation, prolonged the oestrus cycle, and induced persistent vaginal oestrus. The relative weight of the uterus and ovaries was decreased relative to the untreated controls. Squamous cell metaplasia of the epithelial lining of the uterus and cytological changes in the uterus were indicative of chronic oestrogenic stimulation. Ovaries were without corpora lutea, and had degenerative follicles.

MeSH terms

  • Animals
  • Animals, Newborn
  • Estrus / drug effects*
  • Female
  • Genitalia, Female / drug effects*
  • Genitalia, Female / pathology
  • Organ Size / drug effects
  • Polysorbates / toxicity*
  • Rats


  • Polysorbates