Validation of the dual analyte assay of the oestrone:pregnanediol ratio in monitoring ovarian function

Hum Reprod. 1993 Feb;8(2):208-10. doi: 10.1093/oxfordjournals.humrep.a138023.


The purpose of this study was to validate the dual analyte system (Boots Celltech Ltd, Slough, UK) as a marker of ovarian function. For this purpose the urinary profile of the ratio of oestrone-3-glucuronide to pregnanediol-3-glucuronide in urine (E13G/PD3G) was compared with plasma concentrations of oestradiol, progesterone, luteinizing hormone (LH) and follicle stimulating hormone from day 8-24 of the menstrual cycle in 23 women. Daily transvaginal ultrasound was also performed from day 8 of the cycle until sonographic evidence of follicular rupture. The ratio of urinary metabolites exhibited a maximum value close to ovulation. In the majority of cases this coincided with the day of LH peak, and was followed by follicular rupture within the next 24 h in 74% of cases. The assay gives a good marker of ovulation, however, it is unlikely to help in detecting the initiation of the fertile period or the quality of the menstrual cycle.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Enzyme-Linked Immunosorbent Assay
  • Estradiol / blood
  • Estrone / urine*
  • Female
  • Follicle Stimulating Hormone / blood
  • Hormones / blood
  • Humans
  • Luteinizing Hormone / blood
  • Monitoring, Physiologic / methods
  • Ovarian Follicle / diagnostic imaging
  • Ovulation / physiology*
  • Pregnanediol / urine*
  • Progesterone / blood
  • Reproducibility of Results
  • Ultrasonography


  • Hormones
  • Estrone
  • Progesterone
  • Estradiol
  • Luteinizing Hormone
  • Follicle Stimulating Hormone
  • Pregnanediol