Optimizing the biolistic process for different biological applications

Methods Enzymol. 1993;217:483-509. doi: 10.1016/0076-6879(93)17086-k.


The biolistic process is still rapidly evolving. We do not anticipate further major improvements in biolistic apparatus. There will probably still be further major improvements in particles, DNA coating, and vectors, as well as significant further advances in understanding of biological determinants of cell penetration and survival. The technology has currently reached the point at which it can be readily and reliably used for a wide range of applications. Given the information presented in this chapter, new applications can be optimized fairly readily.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bacteriophage lambda
  • Drug Carriers*
  • Equipment Design
  • Escherichia coli
  • Genetic Vectors*
  • Gold
  • Indicators and Reagents
  • Particle Accelerators
  • Plants
  • Transfection / methods*
  • Tungsten / toxicity


  • Drug Carriers
  • Indicators and Reagents
  • Gold
  • Tungsten