Clones of individual repetitive sequences from sea urchin DNA constructed with synthetic Eco RI sites

R H Scheller; T L Thomas; A S Lee; W H Klein; W D Niles; R J Britten; E H Davidson

doi:10.1126/science.847467

Clones of individual repetitive sequences from sea urchin DNA constructed with synthetic Eco RI sites

Science. 1977 Apr 8;196(4286):197-200. doi: 10.1126/science.847467.

Authors

R H Scheller, T L Thomas, A S Lee, W H Klein, W D Niles, R J Britten, E H Davidson

PMID: 847467
DOI: 10.1126/science.847467

Abstract

Interspersed repetitive sequences were isolated from sea urchin DNA by renaturing to low Cot followed by treatment with nuclease SI. Synthetic Eco RI sites were ligated onto the repetitive sequence elements, which were then inserted at the Eco RI site of plasmid RSF2124 and cloned. The repetitive sequences can be excised from the plasmid with Eco RI for further study.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Base Sequence
DNA Restriction Enzymes / metabolism
DNA, Recombinant / analysis*
Molecular Weight
Nucleic Acid Renaturation
Oligodeoxyribonucleotides / chemical synthesis
Polynucleotide Ligases / metabolism
Sea Urchins / analysis*

Substances

DNA, Recombinant
Oligodeoxyribonucleotides
DNA Restriction Enzymes
Polynucleotide Ligases