Structure and expression of novel protein-tyrosine kinases, Emb and Emt, in hematopoietic cells

Biochem Biophys Res Commun. 1993 Apr 15;192(1):231-40. doi: 10.1006/bbrc.1993.1404.


Two novel tyrosine kinase cDNAs were obtained from murine mast cells. These kinases, Emb and Emt, constitute a novel tyrosine kinase subfamily which may also include Tec, a kinase preferentially expressed in liver, and Dsrc28, a fruit fly kinase. Both lack hydrophobic stretches characteristic of the transmembrane domains found in growth factor receptor tyrosine kinases and carboxyl-terminal, negative regulatory tyrosine residue found in Src family kinases. In addition to the Src homology region 2 (SH2) and SH3 domains characteristic of the Src family kinases and other signaling molecules, Emb and Emt share a similar amino-terminal domain comprised mainly of two repeat segments. The emb 2.7-kb transcript was expressed in mast cells, myeloid cells and B lymphocytes while the emt 4.6-kb mRNA in mast cells, myeloid cells and T lymphocytes. The evidence for in vitro tyrosine kinase activity of Emb and Emt proteins is also provided.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cells, Cultured
  • DNA, Single-Stranded
  • Mast Cells / enzymology*
  • Mice
  • Molecular Sequence Data
  • Phosphorylation
  • Protein-Tyrosine Kinases / biosynthesis
  • Protein-Tyrosine Kinases / chemistry*
  • Protein-Tyrosine Kinases / genetics
  • RNA, Messenger / metabolism
  • Sequence Homology, Amino Acid
  • Tyrosine / metabolism


  • DNA, Single-Stranded
  • RNA, Messenger
  • Tyrosine
  • emb protein-tyrosine kinase
  • Protein-Tyrosine Kinases
  • emt protein-tyrosine kinase

Associated data

  • GENBANK/D25302
  • GENBANK/L10627
  • GENBANK/L10628
  • GENBANK/S64630
  • GENBANK/S64631
  • GENBANK/S64632
  • GENBANK/S64635
  • GENBANK/S64636
  • GENBANK/S64637
  • GENBANK/Z19151