Tumor necrosis factor-alpha increased the integrin alpha 2 beta 1 expression and cell attachment to type I collagen in human dermal fibroblasts

Biochem Biophys Res Commun. 1993 Apr 15;192(1):281-7. doi: 10.1006/bbrc.1993.1411.


Cell adhesion molecules of human dermal fibroblasts play an important role in the processes of wound healing. The effects of tumor necrosis factor- alpha (TNF) on the expression of integrin beta 1 subfamily in human dermal fibroblasts were examined. TNF preferentially induced the expression of alpha 2 beta 1 integrins, receptors for collagen and laminin, in a time and dose dependent manner. Cell attachment to type I collagen increased by the treatment with TNF. However, cell attachment to fibronectin and laminin was not increased. This TNF-induced cell attachment could be reduced significantly by anti-integrin alpha 2 beta 1 antibody. Antibodies against receptors other than alpha 2 beta 1 integrin did not significantly reduce cell attachment. These data suggest that the increased attachment of human dermal fibroblasts to type I collagen appears to be mediated predominantly through the augmentation of integrin alpha 2 beta 1 expression by TNF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Cell Adhesion / drug effects
  • Cells, Cultured
  • Collagen / metabolism*
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • Fibronectins / metabolism
  • Humans
  • Integrins / immunology
  • Integrins / metabolism*
  • Laminin / metabolism
  • Skin / cytology
  • Skin / metabolism*
  • Tumor Necrosis Factor-alpha / pharmacology*


  • Antibodies, Monoclonal
  • Fibronectins
  • Integrins
  • Laminin
  • Tumor Necrosis Factor-alpha
  • Collagen