Apoptosis of lung cancer cells caused by some anti-cancer agents (MMC, CPT-11, ADM) is inhibited by bcl-2

Biochem Biophys Res Commun. 1993 Apr 15;192(1):30-6. doi: 10.1006/bbrc.1993.1377.


To determine whether the apoptotic cell death induced by anti-cancer agents could be inhibited by bcl-2, we established a bcl-2-transfected human small cell lung cancer cell line, SBC-3/Bcl2. SBC-3/Bcl2 showed higher resistance to ADM, CPT-11 and MMC compared with the parental line SBC-3, with relative resistance values of 3.4, 7.6 and 5.7, respectively. However, there was no difference in sensitivity to CDDP, VP-16, ACNU, MTX and taxol between SBC-3 and SBC-3/Bcl2. Agarose gel electrophoresis showed typical DNA fragmentation of SBC-3 following treatment with CPT-11 or MMC, in a concentration-dependent manner. In contrast, the same concentration of the drugs did not induce DNA fragmentation in SBC-3/Bcl2. Treatment with CDDP resulted in the same degree of DNA fragmentation in SBC-3 and SBC-3/Bcl2. These studies indicate that bcl-2 can modulate the cytotoxicity of some anti-cancer agents by inhibiting the process of apoptosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents, Phytogenic / antagonists & inhibitors
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Base Sequence
  • Camptothecin / analogs & derivatives*
  • Camptothecin / antagonists & inhibitors
  • Camptothecin / pharmacology
  • Cell Death / drug effects
  • DNA Damage
  • DNA, Neoplasm / drug effects
  • Doxorubicin / antagonists & inhibitors
  • Doxorubicin / pharmacology*
  • Humans
  • Irinotecan
  • Lung Neoplasms / pathology*
  • Mice
  • Mitomycin / antagonists & inhibitors
  • Mitomycin / pharmacology*
  • Molecular Sequence Data
  • Proto-Oncogene Proteins / physiology*
  • Proto-Oncogene Proteins c-bcl-2
  • Transfection
  • Tumor Cells, Cultured


  • Antineoplastic Agents, Phytogenic
  • DNA, Neoplasm
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • Mitomycin
  • Irinotecan
  • Doxorubicin
  • Camptothecin