Accumulation of factor F395 in nifNE mutants of Klebsiella pneumoniae

Can J Microbiol. 1993 Mar;39(3):351-4. doi: 10.1139/m93-050.

Abstract

The nifNE gene products of Klebsiella pneumoniae are required for the in vivo and in vitro synthesis of the iron-molybdenum cofactor (FeMo-co) of nitrogenase. Derepression of nifNE mutants for nitrogenase resulted in the accumulation of a small molecule, factor F395. Factor F395 is protein associated in vivo. We report here initial spectral characterization of this factor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Biological Factors / chemistry
  • Biological Factors / isolation & purification
  • Biological Factors / metabolism*
  • Coenzymes*
  • Enzyme Repression
  • Genes, Bacterial*
  • Klebsiella pneumoniae / genetics
  • Klebsiella pneumoniae / metabolism*
  • Metalloproteins / metabolism*
  • Molybdenum Cofactors
  • Mutation
  • Nitrogen Fixation / genetics
  • Nitrogenase / metabolism*
  • Pteridines / metabolism*
  • Species Specificity

Substances

  • Biological Factors
  • Coenzymes
  • Metalloproteins
  • Molybdenum Cofactors
  • Nitrogenase
  • Pteridines
  • molybdopterin