CaBP2 Is a Rat Homolog of ERp72 With Proteindisulfide Isomerase Activity

Eur J Biochem. 1993 Apr 15;213(2):789-95. doi: 10.1111/j.1432-1033.1993.tb17821.x.

Abstract

Ca-binding protein 2 (CaBP2) has been described previously as an intracisternal calcium-binding microsomal glycoprotein. We report now the primary sequence of this protein as deduced from the corresponding cDNA. The protein possesses a C-terminal -KEEL retention sequence and three repeats of the thioredoxin-like motive -EFYAPNCGHCK-, and represents the rat homolog of ERp72. In contrast to earlier reports on ERp72, CaBP2 possesses significant proteindisulfide isomerase activity. Furthermore, in contrast to ERp72, CaBP2 is a glycoporotein containing O-linked glycans. The amount of CaBP2 in H-35 Reuber hepatoma cells increases in parallel with that of immunoglobin heavy-chain-binding protein under conditions which lead to impaired glycosylation, while the amount of calreticulin, another KDEL-containing glycoprotein, remains almost unchanged.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Calcium-Binding Proteins / genetics*
  • Calcium-Binding Proteins / isolation & purification
  • Calcium-Binding Proteins / metabolism*
  • Cloning, Molecular
  • Gene Library
  • Insulin / metabolism
  • Isomerases / genetics*
  • Isomerases / isolation & purification
  • Isomerases / metabolism*
  • Kinetics
  • Liver / physiology
  • Liver Neoplasms, Experimental / metabolism*
  • Male
  • Membrane Glycoproteins / genetics*
  • Methionine / metabolism
  • Microsomes, Liver / metabolism*
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Protein Disulfide-Isomerases
  • Rats
  • Rats, Wistar

Substances

  • Calcium-Binding Proteins
  • Insulin
  • Membrane Glycoproteins
  • Oligodeoxyribonucleotides
  • endoplasmic reticulum glycoprotein p72
  • Methionine
  • Isomerases
  • Protein Disulfide-Isomerases

Associated data

  • GENBANK/D14867
  • GENBANK/D14868
  • GENBANK/L09679
  • GENBANK/L09680
  • GENBANK/L09681
  • GENBANK/L09682
  • GENBANK/L09683
  • GENBANK/M86870
  • GENBANK/X57950
  • GENBANK/X71000