Staphylococcus aureus binding to cardiac endothelial cells is partly mediated by a 130 kilodalton glycoprotein

J Lab Clin Med. 1993 May;121(5):675-82.

Abstract

Binding of Staphylococcus aureus to vascular endothelial cells may be an initiating event in the pathogenesis of endovascular infection, particularly infective endocarditis. In this study a competition assay between labeled and unlabeled bacteria was used to identify potential S. aureus-binding determinants on the surface of cultured porcine aortic valve endothelial cells. Concanavalin A inhibited 30% to 40% of the specific binding of S. aureus to membrane-associated components. Concanavalin A affinity chromatography of radiolabeled cell-surface proteins, followed by isolation of S. aureus-binding proteins, identified a 130,000-molecular-weight cell surface protein that may function as an endothelial cell S. aureus receptor. These data suggest that specific binding of S. aureus to cardiac valve endothelial cells is mediated in part by a 130 kd mannose-containing glycoprotein.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Aortic Valve / microbiology*
  • Bacterial Adhesion*
  • Cells, Cultured
  • Concanavalin A / metabolism
  • Concanavalin A / pharmacology
  • Endothelium, Vascular / microbiology*
  • Membrane Glycoproteins / isolation & purification
  • Membrane Glycoproteins / physiology*
  • Staphylococcus aureus / pathogenicity*
  • Swine

Substances

  • Membrane Glycoproteins
  • Concanavalin A