There was a considerable change in the structural parameters of both the lignocellulosic substrates. This change was confirmed by infrared (IR) spectroscopy and X-ray diffraction data, which revealed the presence of phenolic groups in the peaks range 1310-1410 and 1200 cm-1 with large crystalline substrates. These were found to be reduced after treatment with alkali. Enzyme/s activity were found to be increased after pretreatment of hydrolysed substrates. The structural modifications in both the substrates were further evidenced by the results obtained after enzymatic hydrolysis of alkali-treated substrates.