Modulation of transferrin receptor expression by insulin and granulocyte-macrophage colony stimulating factor in AML-193 leukemic cells

Cancer Lett. 1993 Apr 15;69(1):51-7. doi: 10.1016/0304-3835(93)90032-5.

Abstract

The effects of insulin and granulocyte-macrophage colony stimulating factor (GM-CSF) on the expression of transferrin receptors in AML-193 myeloid leukemia cell line were investigated. Insulin, alone or in combination with GM-CSF, caused an increase in surface transferrin receptors within 5 min. This increase was inhibited by hyperosmolarity but not by cycloheximide suggesting the involvement of receptor recycling from internal pools. In contrast, receptor increase after 18 h of incubation with insulin and GM-CSF was sensitive to cycloheximide indicating that long term effects of these growth factors are mediated through protein synthesis. These results may have implications on combined GM-CSF + antitransferrin receptor antibody therapy of leukemias.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology*
  • Humans
  • Insulin / pharmacology*
  • Leukemia, Myeloid / metabolism*
  • Receptors, Transferrin / drug effects
  • Receptors, Transferrin / metabolism*
  • Tumor Cells, Cultured

Substances

  • Insulin
  • Receptors, Transferrin
  • Granulocyte-Macrophage Colony-Stimulating Factor