Total numbers of neurons in myenteric ganglia of the guinea-pig small intestine

Cell Tissue Res. 1993 Apr;272(1):197-200. doi: 10.1007/BF00323587.

Abstract

Two techniques that are thought to stain all of the neurons in the myenteric ganglia of the intestine are NADH diaphorase histochemistry and immunohistochemistry using a "nerve cell body" antiserum. However, this assumption has never been directly verified. In the present study myenteric ganglia of the guinea-pig ileum were prepared as whole-mounts and stained with either of these techniques. All nerve cells that could be identified in the whole-mounts were counted. The whole-mounts were then embedded flat in resin and serially sectioned at 1 micron. Nerve cells were identified and counted from the serial sections, and the data compared to those obtained from the whole-mounts. NADH diaphorase histochemistry did not reveal all the neurons at incubation times that gave selective staining. In contrast, "nerve cell body" antiserum stained the entire neuronal population. To determine the total number of nerve cell bodies/ganglion and the proportion of nerve cell bodies with calbindin immunoreactivity, whole-mounts that had been processed for calbindin immunohistochemistry were serially sectioned and reconstructed. The total number of neurons per myenteric ganglion was 105 +/- 10 (SE). Calbindin-immunoreactive neurons comprised about 20% of the myenteric neurons, which is considerably less than previous estimates, because previously the total population has been underestimated. The spatial density of myenteric neurons in the undistended ileum of the guinea-pig is 17,300 nerve cells/cm2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calbindins
  • Cell Count
  • Dihydrolipoamide Dehydrogenase / analysis
  • Ganglia, Sympathetic / enzymology
  • Ganglia, Sympathetic / ultrastructure*
  • Guinea Pigs
  • Immune Sera / immunology
  • Immunohistochemistry
  • Intestine, Small / innervation*
  • Intestine, Small / ultrastructure
  • Myenteric Plexus / enzymology
  • Myenteric Plexus / ultrastructure*
  • Neurons / enzymology
  • Neurons / immunology
  • Neurons / ultrastructure*
  • S100 Calcium Binding Protein G / analysis

Substances

  • Calbindins
  • Immune Sera
  • S100 Calcium Binding Protein G
  • Dihydrolipoamide Dehydrogenase