Investigations carried out on trematode parasites affecting cattle and buffaloes in Ismailia Governorate revealed a degree of infection with Fasciola sp. attaining 6.95% among the former, and 16.16% among the latter. Concerning Paramphistomum sp. infection, it was 43.83% among cattle and 9% among buffaloes. During these studies, it appeared that cattle or buffaloes having no infection with Fasciola sp. or those being infected with Paramphistomum sp. only proved to harbour Entamoeba bovis trophozoites with a percentage of about 85% in cattle, and 80% among buffaloes. This protozoan parasite was also recovered from the gall bladders and bile ducts of slaughtered beef cattle that had no infection with liver flukes. The morphological picture of the fresh E. bovis trophozoite as well as its cyst were described, and the significance of such parasite on its host was discussed.