Development and validation of a solvent extraction technique for determination of Cu-PTSM in blood

Nucl Med Biol. 1993 Apr;20(3):343-9. doi: 10.1016/0969-8051(93)90057-2.


The partitioning of [67Cu]Cu-PTSM between plasma and red blood cells (RBC) was investigated in vitro with human, rat, pig and dog blood. Significant inter-species variability is observed in the plasma/RBC partitioning of tracer, ranging from c. 75% association with plasma in human blood to only c. 35% association with plasma in dog blood. This inter-species difference results from selective association of the [67Cu]Cu-PTSM tracer with human albumin. When [67Cu]Cu-PTSM is mixed with human blood in vitro at 37 degrees C the fraction of 67Cu-radioactivity that remains plasma-associated decreases with time, apparently due to the expected intracellular decomposition of the Cu-PTSM complex by RBC; however, this process is sufficiently slow that it should have limited influence on [62Cu]CU-PTSM biodistribution following intravenous injection. Octanol extraction of blood was found to be an effective technique for quantitating the amount of intact [67Cu]Cu-PTSM complex in blood samples. When imaging with [62Cu]Cu-PTSM, octanol extraction may be useful for determining the [62Cu]Cu-PTSM content of arterial blood samples to establish a true radiotracer input function.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Copper Radioisotopes
  • Dogs
  • Erythrocytes / metabolism
  • Humans
  • Male
  • Organometallic Compounds / blood*
  • Organometallic Compounds / pharmacokinetics
  • Rats
  • Rats, Sprague-Dawley
  • Solvents
  • Swine
  • Thiosemicarbazones / blood*
  • Thiosemicarbazones / pharmacokinetics
  • Tissue Distribution


  • Copper Radioisotopes
  • Organometallic Compounds
  • Solvents
  • Thiosemicarbazones
  • copper (II) pyruvaldehyde bis(N(4)-dimethylthiosemicarbazone)