Analysis of autoantibody binding to 52-kd paraneoplastic cerebellar degeneration-associated antigen expressed in recombinant proteins

Ann Neurol. 1993 Apr;33(4):373-80. doi: 10.1002/ana.410330407.


A 52-kd neural antigen was reported to be recognized by anti-Purkinje cell antibodies in serum of a patient with paraneoplastic cerebellar degeneration associated with uterine carcinoma. In this study, we demonstrate that this neural antigen is recognized by antibodies known as anti-Purkinje cell antibody type I (PCAb Type I) and anti-YO. The latter's antigen is reported to be specific for the 62- to 64-kd antigen CDR62. Assuming that the 52-kd and 62- to 64-kd antigens share a common epitope(s) recognized by all of these antibodies, we examined the antigenic region on the 52-kd protein by immunoblots with deletion fragment proteins of the recombinant 52-kd protein. A major epitope was localized in the region of amino acid residues 94 to 133 of the 52-kd protein, which is the site of a leucine zipper motif. The potential pathogenicity of PCAb Type I is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigens / analysis*
  • Antigens / chemistry
  • Antigens / genetics
  • Autoantibodies / immunology*
  • Cerebellar Diseases / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Immunoblotting
  • Molecular Sequence Data
  • Molecular Weight
  • Paraneoplastic Syndromes / immunology*
  • Peptide Fragments / immunology
  • Recombinant Proteins


  • Antigens
  • Autoantibodies
  • Peptide Fragments
  • Recombinant Proteins