Cell surface glycoproteins expressed on activated human T cells induce production of interleukin-1 beta by monocytic cells: a possible role of CD69

Eur Cytokine Netw. 1993 Jan-Feb;4(1):15-23.


In many immunoinflammatory diseases, macrophages, by producing interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF alpha), stimulate protease secretion in fibroblasts, thus contributing to tissue destruction. Monocyte/macrophage activation is prompted by soluble factors released by activated T cells as well as by cell-cell contact. Indeed, previous studies have shown that monocytes exposed to paraformaldehyde (PFA)-fixed, activated T cells produced high amounts of IL-1 beta. In this report, we used the T cell line HUT-78 to further characterize the T cell factor(s) responsible for monocyte activation by cell-cell contact. After subcellular fractionation, most of the activity was found in the cellular membrane fraction of PHA/PMA-stimulated HUT-78 cells, and proved to be due to glycoproteins, following trypsin digestion and tunicamycin treatment. HUT-78 cells acquired the capacity to stimulate monocytic cells after as little as 1h of stimulation. De novo protein synthesis was required for the expression of the IL-1 beta inducing factor, as shown by cycloheximide treatment. When membrane proteins of PHA/PMA-stimulated HUT-78 cells were separated on SDS-polyacrylamide gel, a peak of stimulatory activity was observed at Mr--25-35 x 10(3). By using specific cytokine inhibitors or blocking mAbs, we ascertained that cell-associated cytokines (IL-1, IL-2, IFN gamma and GM-CSF) were not involved in monocyte activation by cell contact. Anti-CD2 and -CD11a (LFA-1) mAbs partially blocked IL-1 beta production by -25% and -35%, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Antigens, CD / immunology*
  • Cell Line
  • Cytokines / antagonists & inhibitors
  • Humans
  • Interleukin-1 / biosynthesis*
  • Lymphocyte Activation*
  • Membrane Glycoproteins / physiology*
  • Molecular Weight
  • Monocytes / drug effects
  • Monocytes / immunology*
  • Monocytes / metabolism
  • Phytohemagglutinins
  • Subcellular Fractions / metabolism
  • T-Lymphocytes / immunology*
  • Tetradecanoylphorbol Acetate
  • Tumor Cells, Cultured


  • Antibodies, Monoclonal
  • Antigens, CD
  • Cytokines
  • Interleukin-1
  • Membrane Glycoproteins
  • Phytohemagglutinins
  • Tetradecanoylphorbol Acetate