A pathogen-induced gene of barley encodes a HSP90 homologue showing striking similarity to vertebrate forms resident in the endoplasmic reticulum

Plant Mol Biol. 1993 Mar;21(6):1097-108. doi: 10.1007/BF00023606.

Abstract

The full-length nucleotide sequence of a barley (Hordeum vulgare L.) leaf mRNA, found to increase rapidly in amount during infection attempts by the powdery mildew fungus (Erysiphe graminis DC. ex Mérat), is reported. The mRNA encodes a polypeptide of 809 amino acid residues which, by sequence comparison, was identified as a member of the 90 kDa heat shock protein (HSP90) family. The encoded protein most resembles the endoplasmic reticulum (ER) resident HSP90 protein, the 94 kDa glucose-regulated protein (GRP94) of vertebrates, as it possesses both the characteristic N-terminal domain including a signal peptide sequence and the C-terminal ER retention signal (Lys-Asp-Glu-Leu). A transcript cross-hybridizing at high stringency accumulated rapidly in leaves upon heat shock treatment. Genomic DNA blot analysis indicated the presence of a family of related genes in the barley genome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • DNA
  • Endoplasmic Reticulum / metabolism*
  • Genes, Plant*
  • Heat-Shock Proteins / genetics*
  • Hordeum / genetics*
  • Hordeum / microbiology
  • Molecular Sequence Data
  • Plant Proteins / genetics*
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Transcription, Genetic
  • Vertebrates / genetics

Substances

  • Heat-Shock Proteins
  • Plant Proteins
  • DNA

Associated data

  • GENBANK/X67960