Proteins Encoded by an Auxin-Regulated Gene Family of Tobacco Share Limited but Significant Homology With Glutathione S-transferases and One Member Indeed Shows in Vitro GST Activity

Plant Mol Biol. 1993 Mar;21(6):965-72. doi: 10.1007/BF00023595.

Abstract

A number of cDNAs corresponding to auxin-regulated mRNAs have been isolated from tobacco and found to be encoded by a multigene family consisting of three subfamilies. Homologous proteins have been isolated independently from soybean and potato. Here we report that the encoded proteins show a limited but significant homology to both plant and animal glutathione S-transferases (GST, EC 2.5.1.18). For the protein NT103, encoded by a member of the Nt103 subfamily, we demonstrate an in vitro GST activity. This is the first time a function is attributed to a member of this group of auxin-induced proteins or any of its homologues. The implications of this finding and the possible relationships between auxins and GSTs are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Dinitrochlorobenzene / metabolism
  • Gene Expression Regulation*
  • Gene Expression Regulation, Enzymologic
  • Glutathione Transferase / genetics*
  • Glutathione Transferase / metabolism
  • Indoleacetic Acids / physiology
  • Molecular Sequence Data
  • Multigene Family*
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Plants, Toxic*
  • Sequence Homology, Amino Acid
  • Tobacco / enzymology
  • Tobacco / genetics*

Substances

  • Indoleacetic Acids
  • Plant Proteins
  • Glutathione Transferase
  • Dinitrochlorobenzene