Analysis of phenolic and flavonoid compounds in juice beverages using high-performance liquid chromatography with coulometric array detection

J Chromatogr. 1993 Apr 9;635(1):143-50. doi: 10.1016/0021-9673(93)83125-c.

Abstract

Analysis of phenolics and flavonoids in juice beverages using reversed-phase HPLC with coulometric array detection is described. Sixteen serial coulometric detectors were used for on-line resolution of co-eluting compounds and generation of voltammetric data. Within each class of compounds, oxidation potential corresponded to specific substitution patterns where: catechol < methoxycatechol < monohydroxyl < methoxyl. Twenty-seven standard compounds were resolved in a 45-min run. The limits of detection were in the low ng/ml range with a linear response range of at least three orders of magnitude. Intra-run retention time variation was < 1% (R.S.D.) and adjacent sensor response ratios varied by < 5% (R.S.D.). The utility of this technique in generating multivariate data for differentiation of juices and juice mixtures is shown.

MeSH terms

  • Analysis of Variance
  • Beverages / analysis*
  • Chromatography, High Pressure Liquid
  • Citrus / chemistry
  • Electrochemistry
  • Flavonoids / analysis*
  • Fruit / chemistry*
  • Phenols / analysis*
  • Reference Standards

Substances

  • Flavonoids
  • Phenols