Differences in the collagen types synthesized by lapine articular chondrocytes in spinner and monolayer culture

Arthritis Rheum. 1977 Mar;20(2):709-16. doi: 10.1002/art.1780200211.


In five different secondary monolayer cultures of rabbit articular chondrocytes, 72-89% of the the collagen synthesized was Type 1, as determined by alpha chain separation and CNBr-cleavage peptide analysis. When sister cells were transferred to spinner bottles after primary monolayer culture growth, 88% of the collagen formed in four separate experiments was Type II. A reversion to Type I collagen synthesis occurred when the spinner-cultured cells were returned to monolayer flasks. The change in the species of collagen depended on the suspension condition and not on the low Ca2+ content (0.33 mM) of the spinner medium. These findings parallel the switch to phenotypic glycosaminoglycan synthesis that also occurs when monolayer-cultured cells are transferred to spinner bottles. Whether this phenomenon arises through a genetic control mechanism rather than environmental selection for particular cohorts of cells has not yet been determined.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cartilage, Articular / cytology*
  • Cartilage, Articular / metabolism
  • Collagen / analysis
  • Collagen / biosynthesis*
  • Rabbits


  • Collagen